P. guajava leaf extracts prevent intestinal colonization of C. rodentium in the mouse model
Principal Investigator Dr. Tannaz Birdi
Project Team Dr. Pooja Gupta
Funder Corpus Grant to FMR from Jamsetji Tata Trust
Diarrhoeal diseases are the second highest cause of mortality of children under 5 years worldwide. There is a continuous search for developing a cost effective treatment for diarrhoea as the present ones are facing challenges. Medicinal plants have been screened as an alternative treatment for diarrhoea. Use of P. guajava leaves as an antidiarrhoeal has been demonstrated globally. Previous studies performed at FMR have proved its in vitro efficacy. Preclinical in vivo biological screening is important since some compounds which show activity in vitro may be metabolized to inactive metabolites in vivo. On the other hand, some extracts may only show in vivo activity due to the metabolism of inactive compounds into active forms. Citrobacterrodentium, a common mouse pathogen, is known to mimic the pathogenecity of enteropathogenic and enterohaemorrhagicE. coli. It can thus present an effective model to study infectious diarrhoea. In the present study P. guajava leaf extract was tested for its efficacy in treating infectious diarrhoea using C. rodentium mouse model.
Key findings & conclusion (Gupta et al., 2014)
The bacterial load in the fecal sample of the mice in the test group was high from day 4 to day 10 as compared to that in the control group suggesting a flush out of the bacteria.
In the test group, 6/7 mice showed clearance of infection by day 19. All mice in the control group continued to show infection till Day 29.
P. guajava leaf extract thus has the potential for use in treatment of infectious diarrhoea.